T4 DNA Ligase Promega
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T4 DNA Ligase - Promega CorporationJoins Two DNA Strands with Cohesive or Blunt Ends. Use with 5´, 3´ or blunt-ended dsDNA (e.g., inserts and vectors); Qualified for blue/white cloning, ... | [PDF] T4 DNA Ligase Protocol - Promega Corporationall Promega products to prevent direct human contact. ... Enzyme Storage Buffer: T4 DNA Ligase is supplied in 10mM Tris-HCl (pH 7.4), 50mM KCl, 1mM DTT, ... twVersatile Zero Background T-Vector System for Gene Cloning and ...2009年4月29日 · (2) Ligation of XcmI-digested pGXT with the PCR product of the rice blast fungus M. oryzae gene MGG_07986.5 using T4 DNA ligase from Promega. (3) ...Promega T4 DNA Ligase - Modifying Enzymes - Fisher ScientificFor ligation of DNA ends. Shop Promega T4 DNA Ligase at Fishersci.com. | [PDF] T and pGEM(R)-T Easy Vector Systems Technical Manual #TM042Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA ... Use only Promega T4 DNA Ligase supplied with this system to perform.Cloning with pGEM®-T Vectors: Ligation - Promega Connections2020年1月8日 · The insert is directly ligated to the T-tailed plasmid vector with T4 DNA ligase. The insert can then be easily transferred from the T ...T4 DNA Ligase (Rapid) Protocol - Sigma-AldrichT4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5' phosphate and a 3' hydroxyl groups of duplex DNA or RNA.T4 DNA Ligase - Takara BioT4 DNA Ligase is a ligation enzyme that can be used to join DNA fragments by catalyzing the formation of phosphodiester bonds between juxtaposed 5' ...T4 DNA Ligase from Promega | Biocompare.comT4 DNA Ligase catalyzes the joining of two strands of DNA between the 5'-phosphate and the 3'-hydroxyl groups of adjacent nucleotides in either a ... tw圖片全部顯示
延伸文章資訊
- 1Blunt-end cloning method protocol
a challenge due to the inefficient ligation of blunt ends by T4 DNA ligase. This protocol demonst...
- 2DNA Insert Ligation (sticky-end and blunt-end) into Vector DNA
T4 DNA Ligase. 5 U. Water, nuclease-free to 20 μL. Total volume. 20 μL. This protocol is for the ...
- 3Protocol - How to Ligate Plasmid DNA - Addgene
Protocol: Standard Insert + Vector DNA Ligation ... the length of the DNA to get a proper ratio o...
- 4T4 DNA Ligase Protocol - Promega Corporation
Blunt-end ligations generally are efficient at temperatures between 15–20°C for 4–18 hours, while...
- 5A Beginner's Guide to How Blunt-End Cloning Works
Now let's talk about how blunt-end cloning works. Basically, the blunt-ended insert and linearize...